Action of pure phospholipase A2 and phospholipase C on human erythrocytes and ghosts
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چکیده
منابع مشابه
Involvement of cytosolic phospholipase A2 and secretory phospholipase A2 in arachidonic acid release from human neutrophils.
The purpose of this study was to define the role of secretory phospholipase A2 (sPLA2), calcium-independent PLA2, and cytosolic PLA2 (cPLA2) in arachidonic acid (AA) release from fMLP-stimulated human neutrophils. While fMLP induced the release of extracellular sPLA2 activity and AA, 70% of sPLA2 activity remained associated with the cell. Treatment with the cell-impermeable sPLA2 inhibitors DT...
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Treatment of dog thyroid slices with an enzyme present in the growth medium of Clostridium perjringens makes them unresponsive to thyroid-stimulating hormone (TSH) as measured by the oxidation of glucose-1J4C to 14C02. This enzyme appears to be phospholipase C since (a) both the anti-TSH effect and the phospholipase activity are inseparable over a 240-fold purification, (b) both activities are ...
متن کاملBimodal regulatory effect of melittin and phospholipase A2-activating protein on human type II secretory phospholipase A2.
Melittin and phospholipase A2-activating protein (PLAP) are known as efficient activators of secretory phospholipase A2(sPLA2) types I, II, and III when phospholipid liposomes are used as substrate. The present study demonstrates that both peptides can either inhibit or activate sPLA2 depending on the peptide/phospholipid ratio when erythrocyte membranes serve as a biologically relevant substra...
متن کاملInhibition of phospholipase A2.
Phospholipases A2 are involved in inflammatory processes such as the liberation of free arachidonic acid from the membrane pool for the biosynthesis of eicosanoids. Inhibitors of these enzymes are proving useful in determining the biological roles of phospholipases A2 in complex cellular processes and may also have therapeutic potential. Inhibition of these lipolytic enzymes is more difficult t...
متن کاملCarvacrol attenuates serum levels of total protein, phospholipase A2 and histamine in asthmatic guinea pig
Objective: Pharmacological effects of carvacrol such as its anti-inflammatory activities have been shows. In this study the effects of carvacrol on serum levels of total protein (TP), phospholipase A2 (PLA2) and histamine in sensitized guinea pigs was evaluated. Materials and Methods: Sensitized guinea pigs were given drinking water alone (group S), drinking water containing three concentration...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Biomembranes
سال: 1971
ISSN: 0005-2736
DOI: 10.1016/0005-2736(71)90024-1